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            gӭRϺZƼ̳
            rg9:00-18:00
            ȫ՟ᾀ13564515386

            ~ͶO-DøDGATELISAzyԇ

            ӆُ
            [lr]
            Ҏ
            r
            Mӆُᾀ13564515386

             

            O-DøDGATELISAzyԇ

            ʹf

            zyԭ

            ԇвpwAķø“ԇ򞣨ELISAAȰO-DøDGAT@wİ΢μ˱˜ƷHRPӛęzyw^صϴõTMB@ɫTMB^øĴ߻D{ɫDKSɫɫ\͘ƷеO-DøDGATPø˃x450nm LœyȣOD ֵӋƷ

            Ʒռ̎淽

            1.  Ѫ壺ʹòԭ̓ȶصԇ^бκμ̼ռѪҺ3000Dx10犌ѪͼtѸСĵطx

            2.  Ѫ{EDTA}ؿ3000Dx30ȡ

            3.  Һ3000Dx10ȥw;ۺ

            4.  M{Mm}ˮv3000Dx10ȡ

            5.  棺ӱռ󲻼rzyՈһb-20ⷴ̓Ҝ½_Ʒسֽ

            ԂƷ

            1. ø˃x450nm

            2. ߾ȼӘ^0.5-10uL2-20uL20-200uL200-1000uL

            3. 37

            ע

            1.  ԇб2-8ʹǰҜƽ20ıȡĝsϴҺнY@FˮԡӟʹYȫܽʹ

            2.  вõİlŻԷܷ⣨͜ظ

            3.  A̎Ęӱoϡֱȡ10μLӘӼ

            4.  fИĕrgҺMМ

            5.  ҺwMʹǰ֓u

            ԇнM

            Q

            96

            48

            ע

            ΢ø˰

            12×8l

            12×4l

            o

            ˜Ʒ

            0.3mL

            0.3mL

            o

            ӱϡҺ

            6mL

            3mL

            o

            zyw-HRP

            10mL

            5mL

            o

            20×ϴ쾏_Һ

            25mL

            15mL

            fMϡ

            A

            6mL

            3mL

            o

            B

            6mL

            3mL

            o

            KֹҺ

            6mL

            3mL

            o

            Ĥ

            2

            2

            o

            f

            1

            1

            o

            Է

            1

            1

            o

            ע˜ƷΞ飺8040201050 U/L.

            ԇĜʂ

             20×ϴ쾏_Һϡጣsˮ120ϡ1ݵ20×ϴ쾏_Һ19ݵsˮ

            ϴ巽

            1.  ֹϴ壺˦M׃Һwÿ׼ӝMϴҺo1min˦M׃Һwˮĸϴ5

            2.  ԄϴCÿעϴҺ350μL1minϴ5

            E

            1.  Ҝƽ20minXȡlʣlԷܷŻ4

            2.  OؘƷ׺͘ӱ˜Ʒ׸ӲͬȵĘ˜Ʒ50μL

            3.  yӱȼӴyӱ10μLټӘӱϡҺ40μL

            4.  S˜Ʒ׺͘ӱÿ׼^øHRPӛęzyw100μL÷Ĥס37ˮԡ偻60min

            5.  ȥҺwˮĸÿ׼ӝMϴҺo1min˦ȥϴҺˮĸ؏ϴ5ΣҲϴCϴ壩

            6.  ÿ׼AB50μL37ܹ15min

            7.  ÿ׼KֹҺ50μL15min450nmL̎y׵ODֵ

             LƘ˜ExcelԘ˜ƷMODֵvLƳ˜ƷԻؚwӋӱֵ

             

            ԇ

            1.  ʴ_ԣ˜ƷԻؚwcAڝPϵRֵڵ0.9900

            2.  `ȣ͙zyС0.1 U/L

            3.  خԣcԽYサ淴

            4.  ؏ԣ׃ϵС10%g׃ϵС15%

            5.  2-8ܹ

            6.  Чڣ6

            ؟•

            1.   ԇЃHоʹRwtaһкɌ߳Г˾Ųؓ؟

            2.   f`fɌ߳Г


            FOR RESEARCH USE ONLY. 

            NOT FOR USE IN DIAGNOSTIC PROCEDURES.

             

            Fish Diacylglycerol O-acyltransferase (DGAT) ELISA Kit instruction

             

            Intended use

            This DGAT ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of DGAT in the sample, this DGAT ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus DGAT concentration. The concentration of DGAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

            Sample collection and storages

            Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20 or -80.Avoid repeated freeze-thaw cycles

            Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8 within 30 minutes of collection. Store samples at -20or -80. Avoid repeated freeze-thaw cycles.

            Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20or -80. Avoid repeated freeze-thaw cycles.

            Note:  The samples should be centrifugated adequately and no hemolysis or granule was allowed.

            Materials required but not supplied

            1.  Standard microplate reader(450nm)

            2.  Precision pipettes and Disposable pipette tips.

            3.  37 incubator

            Precautions

            1.  Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.

            2.  Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.

            3.  Mix all reagents before using.

            Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)

            Materials supplied

            Name

            96 determinations

            48 determinations

            Microelisa stripplate

            12*8strips

            12*4strips

            Standard

            0.3ml

            0.3ml

            Sample diluent

            6.0ml

            3.0ml

            HRP-Conjugate reagent

            10.0ml

            5.0ml

            20X Wash solution

            25ml

            15ml

            Chromogen Solution A

            6.0ml

            3.0ml

            Chromogen Solution B

            6.0ml

            3.0ml

            Stop Solution

            6.0ml

            3.0ml

            Closure plate membrane

            2

            2

            User manual

            1

            1

            Sealed bags

            1

            1

            Note: Standard concentration was followed by:

            8040201050 U/L.

            Reagent preparation

            20×wash solution:Dilute with Distilled or deionized water 1:20.

            Assay procedure

            1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.

            2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.

            3.  Add Sample: Add testing sample 10μl Then add sample diluent 40μl to testing sample well; Blank well doesnt add anyting.

            4.  Add 10l of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 

            5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.

            6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.

            7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not


            appear uniform, gently tap the plate to ensure thorough mixing.

            8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.

            Calculation of results

            1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.

            2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.

            3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.

            4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.

            5. The sensitivity by this assay is 0.1 U/L.

            6. Standard curve

              

            Storage  2-8.

            validity six months.

             

            FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

            |aƷ^|Ʒƌ^|g|˾|ʽ

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